The Effect of Sertoli Cells Feeders on Mouse Spermatogonial Stem Cells Cultured in vitro
- VernacularTitle:Sertoli细胞饲养层对小鼠精原干细胞增殖的影响
- Author:
Hui-Lian LIU
;
Guang-Qiang ZHAO
;
- Publication Type:Journal Article
- Keywords:
Spermatogonial stem cell Sertoli feeders in vitro Mouse
- From:
China Biotechnology
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Spermatogonial stem cells(SSCs)dissociated from 2～5 days postpartum mice were cultured on Sertoli cells feeders,to study the effect of Sertoli cells feeders on culture of mouse spermatogonial stem cells.Methods Specific markers CD9 of mouse SSCs cultured serum-free StemPro-34 SFM culture medium were identified by immunohistochemical assay.Result During the first week of culture,on Sertoli cells feeders or STO feeders,the biologica1 behaviors of spermatogonial stem cells showed no obvious difference.After a week of culture,compared with control,there were more number of spermatogonial stem cells remained when they were cultured for 60 days.These cells were expressed CD9 positive.In conclusion Sertoli cells can be used as feeders not only to promote survival but also renewal of spermatogonial stem cells.
