Screening of Lipase-producing Strain for Catalytic Synthesis of Ester in Organic Media
- VernacularTitle:产有机相催化酯合成活性的脂肪酶菌株的筛选
- Author:
Hong-Ling YUAN
;
Lu-Hong TANG
;
Zheng-Hong XU
;
Wen-Yi TAO
;
- Publication Type:Journal Article
- Keywords:
Organic phrase;
Lipase;
Screening;
Properties
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
An organic solvent tolerant isolate A213 originating from soil samples were successfully isolated via direct plating method using 10g/L of toluene as the sole carbon source and transparent cycle plate assay method.It was identified as Yarrowia based on its characteris- tics.The results in shake flask cultivation showed that the suitable tipase producing media were(g/L):yeast extract 40,vegetable oil 10, MgSO_4?7H_2O1,KH_2PO_4 5.Under optimal culture conditions (27℃and pH 6.5 ),the maximal lipase activity could reach 67.8 IU/ mL The optimal pH and temperature for the hydrolysis of p-nitrophenyl acetate by crude lipase were pH6.5 and 40℃The enzyme was sta- ble under 70℃and pH 5.5～8.5.Then isolate A213 was found to produce the lipase which can synthesize L-ascorbyl palmitate in tert-amyl alcohol validated by the thin-layer chromatography.
