Isolation and Characterization of a Temperature-sensitive p-Nitrophenol Degradation Related Genes Mutant Strain
- VernacularTitle:PNP降解相关基因温敏突变株的分离及其特性研究
- Author:
Hai-Hua DENG
;
Xiao-Dan LI
;
Hui CAO
;
Shi-Ming WANG
;
Ting-Ting HUANG
;
Zhong-Li CUI
;
- Publication Type:Journal Article
- Keywords:
p-Nitrophenol;
Hydroquinone;
Transposon insertion mutant;
Temperature-sensitive mutant
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A temperature-sensitive mutant strain was isolated after transposon mutagenesis with Tn5 and named MT54.PCR was carried out with primers designed according to the sequence of transposon,the PCR products showed that the MT54 carried transposon in the genome. The mutant grew well at 30℃in minimal medium(MM)containing p-nitrophenol(PNP)as sole carbon source,while it cannot grow at 37℃in the same medium,NO_2.detection results also proved that.Comparing the degradation rate of PNP and hydroquinone of MT54 and DLL-E4 at different temperature,it was speculated that the mutant site locate in the PNP degradation related genes.
