Cloning and Alignment of the Partial Mannanase Gene of Bacillus spp
- VernacularTitle:芽胞杆菌?-甘露聚糖酶基因部分序列的克隆及相似性分析
- Author:
Ya-Nan LI
;
Kun MENG
;
Pei-Long YANG
;
Ya-Ru WANG
;
Bin YAO
;
- Publication Type:Journal Article
- Keywords:
Bacillus spp;
?-mannanase gene;
Homology analysis
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
By functional plates,16 strains which can produce?-mannana-se were isolated frnm 28 Bacillus spp.Using a pair of degenerated primers,the conserved fragments of?-mannanase gene from the selected strains were amplified by PCR.The obtained nucleotide fragments were sequenced and compared with the homologous?-mannanase genes in GenBank and a phylogenetic tree was generated.Comparing to the genes coding?-mannanase published,the cloned nucleotide fragments show the highest sequence identity between 62% and 98%.The genes coding fnr?-mannanase of Bacillus circulus have low identity while the?-mannanase genes of Bacillus subtilis and other Bacillus spp. have high identity.
