Comparison with Several Methods to Isolate Epiphytic Bacteria from Gracilaria lemaneiformis (Rhodophyta)
- VernacularTitle:龙须菜体表附生细菌的几种分离方法比较
- Author:
Yong-Jian XU
;
Guan-Zong LE
;
You-Ping ZHANG
;
- Publication Type:Journal Article
- Keywords:
Seaweed;
Gracilaria lemaneiformi;
Epiphytic bacteria;
Isolation method
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
We used 4 methods,such as ultrasonic crush(UC),ultrasonic rinse(UR),whorl surge(WS)and rubbing(RU),to isolate epiphytic bacteria from red alga Gracilaria lemaneiformis.Then,we counted bacteria numbers,detected bacterial species,observed bacterial configuration and characteristic of cell wall.Compared with these methods and with different treatments in one method,the results were drawn:the UR and RU were inferior in all methods to isolate bacterial numbers and species,the UC and WS were better,especially,the treatment 30W 30s of UC was the best in experiment,which isolated 12 of 16 bacterial species,and got 1.75 10~6 cells per gram wet weight G.lemaneiformis.
