Activity Study of Ciliary Neurotrophic Factor(CNTF)Mutant
- VernacularTitle:睫状神经营养因子突变体蛋白的活性研究
- Author:
Hua BI
;
Li-Yong YUAN
;
Xin-Chang SHI
;
Yang ZHAO
;
Lan LIU
;
Chun-Ming RAO
;
Jun-Zhi WANG
;
- Publication Type:Journal Article
- Keywords:
CNTF Mutant Bioactivity
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To study still further the activity of CNTF mutant designed by computer molecular modling,the methods of dissociated cultures of chick dorsal root ganglion、TF-1 prolification and the normal mice'weight loss tests weve used.The results indicated that the mutant protein promoted the survival of dorsal root ganglion、induced TF-1 prolification and made the normal mice lose weight,decrease appetite and reduce fat index.The weight loss effect was dependant with its administration dosage,ED50 was 150.986?g/kg/d.To TF-1,the specific activity reached 2.0?106U/mg against international reference reagent.In a word,CNTF mutant had excel bioactivity.So it provided clues for its development and application.
