Construction of OTX1 Lentiviral Vector and Overexpression Research
- VernacularTitle:OTX1基因慢病毒载体的构建及过表达研究
- Author:
Ping REN
;
Shu-Yan WANG
;
Yun-Qian GUAN
;
Yan-Ling XU
;
Yu ZHANG
;
- Publication Type:Journal Article
- Keywords:
Lentivirus OTX transcriptional factor Gene expression
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
OTX1 gene is one of the pivotal transcriptional factors involved in the neurogenesis.In order to overexpress the OTX1 gene in distinct cell types and find out its contribution to the proliferation and differentiation of stem cells in vitro,OTX1 cDNA was subcloned into lentiviral vectors.The resulting constructions pDUETOTX1,pDUETGFPOTX1 and pDUETGFP were packaged in 293 cells producing viral particles to transduce 293T cells,SY5Y cells,mouse embryonic stem cells and E15 neural stem cells.It was proved that the transferred OTX1 gene was located in the nuclei of the transduced cells in stead of plasma.Lentivirus is an ideal vector delivering gene to different cells.The overexpression of OTX1 in transduced 293T cells were validated by Western blot and immunofluorescence.
