Disruption of hom Gene Encoding for Homoserine Dehydrogenase of Corynebacterium glutamicum
- VernacularTitle:谷氨酸棒杆菌高丝氨酸脱氢酶编码基因hom的敲除
- Author:
Zhi-Ming RAO
;
Jun-Sheng ZHANG
;
Wei SHEN
;
Hui-Ying FANG
;
Jian ZHUGE
;
- Publication Type:Journal Article
- Keywords:
Lysine Homoserine dehydrogenase-disruption Corynebacterium glutamicum
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The hom gene encoding for homoserine dehydrogenase was amplified from the genomic DNA of Corynebacterium glutamicum ATCC 13032.After the kanamycin-resistant gene(Km)cassette from plasmid pET28a was inserted into the center of hom,the hom::Km cassette was then electroporated into the competent cell of C.glutamicum ATCC 13032.And kanamycin-resistant clones were obtained.PCR was performed to confirm whether the Km gene was integrated into the hom gene of these clones and the recombinant strains of hom-disrupted were screened out.Fermentation results showed that the lysine yield of the hom-disrupted strain C.g-hom::Km-8 reached 4.7 g/L,which was 6.7 times that of C.glutamicum ATCC 13032.
