Quantitation of albumin in urine by HPLC and initial clinical application
- VernacularTitle:HPLC法测定尿白蛋白方法的建立和初步临床应用
- Author:
Xiao-Fei CHEN
;
Jian-Rong YANG
;
Li-Ling CHEN
;
Yao-Sheng XIE
;
Huai-Kai WEN
;
Xiao-Fang HUANG
;
Yu-Min WANG
;
- Publication Type:Journal Article
- Keywords:
Albuminuria;
Chromatography,high pressure liquid;
Diabetic nephropathy
- From:
Chinese Journal of Laboratory Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To measure albumin in urine by HPLC and conduct primary clinical application Methods Solvent gradient and appropriate wave length was optimized and performance of the HPLC method was evaluated.Urine albumin of 46 patients with diabetes was measured.Results In standard and urine,retention time of Alb was 13.1 min.The linear measuring range extends to 1 820 mg/L.The lower limit of measurment for Alb was 4.2 mg/L.The intra-assay CV and the inter-assay CV were 3.36%,4.12% and 1.93%,1.97% at 24.5 mg/L and 546.9 mg/L of Alb respectively.Analytical recovery rate were 96.3%,98.2% and 97.5%.Microalbuminuria rate was 54.3% by HPLC,26.1% by immunoassay in 46 patients with diabetes.Conclusions Measurement of Alb in urine by HPLC is feasible as routine method until quantifying urinary total Alb conveniently.HPLC is the same to suit research for diabetic nephropathy and so on.
