The SARS-CoV 3a and 7a Protein May Enhance the Induction of IFN-?
- VernacularTitle:人IFN-?启动子基因报告质粒的构建及SARS-CoV3a和7a蛋白对IFN-?诱生作用的初步研究
- Author:
Chun-E XU
;
Ling FU
;
Lihua HOU
;
ShaoJie WENG
;
DaZhi LAI
;
JianMin LI
;
Ting YU
;
ChangMing YU
;
Wei CHEN
- Publication Type:Journal Article
- Keywords:
SARSCoV 3a protein 7a protein IFN? promoter Luciferase reporter system
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
3a and 7a are nonstructural proteins of SARSCoV, which are encoded separately by ORF 3a and ORF 7a in SARSCoV genome. The expression of 3a has been founded in cells infected by virus in vivo or in vitro. Firstly, the pGL3Control vector was reconstructed , the pGL3Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN? promoter gene was cloned into the pGL3Enhancer vector and pGLIP21, the Luciferase reporter plasmid with IFN? promoter was established. The availability of pGLIP21 was verified by NDV ,the inductor of IFN?, the Luciferase activity was assayed in cells transfected with pGLIP21 by Luminometer. In order to see the function of 3a and 7a protein of SARSCoV,CHO cells expressing 3a or 7a protein were transfected with pGLIP21, the intensity of luciferase activity was analyzed . By analysis, in vitro, 3a and 7a protein of SARSCoV had the similar ability in triggering the expression of Luceferase gene, i.e 3a and 7a protein of SARSCoV could effectively activate the promoter fragment of IFN? gene. This result will help studying the function of 3a and 7a protein and provide a method to study the nosogenesis mechanism of SARSCoV.
