Adhesion and Proliferation of Bone Mesenchymal Stem Cells with PLGA-[ASP-PEG] Polymer Scaffolds
- VernacularTitle:BMSCs在PLGA-[ASP-PEG]基质材料表面粘附及增殖的研究
- Author:
Zhi-Xia DUAN
;
Qi-Xin ZHENG
;
Xiao-Dong GUO
;
- Publication Type:Journal Article
- Keywords:
Biomaterial tri-block polymer scaffold Adhesion Proliferation Bone mesenchymal stem cells Tissue engineering
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To investigate the effects of adhesion and proliferation of bone mesenchymal stem cells (BMSCs) in the surface of lactic acid/glycolic acid/asparagic acid-co-polyethylene glycol PLGA-[ASP-PEG] tri-block polymer scaffolds, try to find a new biomaterial to induce seed cells in vitro for bone tissue engineering. Methods: Modified PLGA with polyethylene glycol (PEG) and asparagic acid (ASP) that has many ligands, and synthesis PLGA-[ASP-PEG] polymer material. BMSCs were cultured in PLGA-[ASP-PEG] polymer material and PLGA used as control group. Through precipitation method, MTT assay and total cellular protein detection to test the adhersion and proliferation of BMSCs. Scanning electron microscope is used to observe cells appearance. Results: BMSCs on the surface of PLGA-[ASP-PEG] polymer scaffolds are adherention to the culture flask, the number of cells is much higher than PLGA’s. The precipitation method suggest that adhesion and proliferation of BMSCs on the surface of PLGA-[ASP-PEG] is much higher than the control group(P
