Application of Flow Cytometry in Viability Detection of Pichia pastoris Cells
- VernacularTitle:应用流式细胞术检测毕赤酵母的细胞活性
- Author:
An-Feng XIAO
;
Xiang-Shan ZHOU
;
Li ZHOU
;
Yuan-Xing ZHANG
;
- Publication Type:Journal Article
- Keywords:
Flow cytometry,Detection,Pichia pastoris,Cell viability
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Cell viability of Pichia pastoris was detected by flow cytometry (FCM) with two reagents fluorescein diacetate (FDA) and propidium iodide (PI). Compared with FDA/PI double-stained dot plots and PI single-stained dot plots,the latter could divide dead and living cells into two separate zones,and get the correct proportion. Then PI single-stained method was used to detect the change of cell viability in Pichia patoris fermentation. At glycerol batch and fed-batch phase,little dead cells were detected. At methanol fed-batch phase,cell viability decreased when cell weight increased,and was only 73.8% at 88 h.
