Expression Diversity of Quorum-sensing-Related Genes in Pseudomonas aeruginosa
- VernacularTitle:铜绿假单胞杆菌QS相关基因差异表达的研究
- Author:
Yi-Min TONG
;
Xin PAN
;
Guang-Hua RONG
;
Zhong-Tian QI
;
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa,Quorum-sensing,Gene,cDNA chip,Expression diversity
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
One hundred Pseudomonas aeruginosa quorum-sensing-related genes were selected and their primers were synthesized. The fragments of specific sequences which are related QS genes were amplified by PCR. These verified sequences were inserted into the vector pMD-18T for sequencing. These DNA fragments were dotted onto glass slides to make cDNA microarray. Hybridization was performed with cy3/cy5-dCTP labeled probes. The scanning data of early stationary phase and mid-logarithmic phase indicated that 9 genes were up-regulated and 6 genes were down-regulated. Undergoing the different medicines,we took tobramycin as an example to compare the expression diversity. The results confirm that the QS cDNA chip is useful,and may contribute to better understand the mechanism of quorum-sensing,and can help us find the new targets for restraining the growth of Pseudomonas aeruginosa.
