Breeding of High-yielding ?-galactosidase Strains from Protoplast of Aspergillus niger
- VernacularTitle:原生质体诱变选育乳糖酶高产菌株
- Author:
Hai-Ying DU
;
Hong-Wei YU
;
Jun HAN
;
Ning LI
;
Ying-Min JIA
;
- Publication Type:Journal Article
- Keywords:
Aspergillus niger,Protoplast,Lactase,Induced breeding
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The protoplasts of original Aspergillus niger strain Uco-3 were treated with the cooperation of UV and ?-ray to obtain the high-yielding strain producing the thermostable ?-galactosidase. Under the optimum conditions of formation and regeneration protoplasts were prepared. According to the interaction of positive mutation rate and radiation dose,the optimum condition was determined. The optimum dose of UV was 4 minutes and the optimum dose of ?-ray was 500 Gy. After mutagenetic treatment of protoplasts and selection from a lot of mutants,a mutant DL116 producing the thermostable ?-galactosidase was obtained. The ?-galactosidase activity of DL116 was increased from 16.27 U/mL to 44.37 U/mL,which was higher than that of strain Uco-3.
