Improvement and Analysis in Over- siderophores Production Bacteria Filtrating and Detecting
- VernacularTitle:适合高产铁载体细菌筛选、检测体系的改进与探析
- Author:
Xiang ZHAO
;
Zhi-Xiong XIE
;
Shao-Xing CHEN
;
Ping SHEN
;
- Publication Type:Journal Article
- Keywords:
Siderophores,Chrome azurol sulfonate (CAS) assay,Over- siderophores production bacteria
- From:
Microbiology
1992;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
A novel stable blue agar plate which is convenient to preparing and more effective than the universal chrome azurol sulfonate (CAS) assay established by Schwyn and Neilands was designed by replacing MM9 growth medium and pipes with certain concentrate of phosphorus buffer solution which pH could be stabled at 6.8. It is more suitable for screening over- siderophores production bacteria. Since OD_ 630 of the sample is usually out of the range of spectrophotometer with CAS assay solution when quantifying the siderophores and the outcome is not steady,the measuring wavelength had been changed to 680 nm corresponding to the middle of max absorbance and the correlation between siderophores concentrations and OD was unchanged. But the detecting sensitivity is elevated by enlarged the absorbance differences among samples with different productivity of siderophores at 680 nm .
