Tilling in Rice Breeding
- VernacularTitle:TILLING在水稻育种中的应用前景
- Author:
Shuang-Yong YAN
;
Xue-Jun LIU
;
Jing-Ping SU
;
Zhong-You MA
;
Lin-Jing SUN
;
- Publication Type:Journal Article
- Keywords:
TILLING Rice Germplasm identification EMS mutagenesis
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Targeting induced local lesions in genomes (TILLING) is a reverse genetics method for functional genomics research.It is possible to screen for point mutations in the populations of EMS mutagenesis with highthroughput and lowcost. EcoTILLING a method based on TILLING ,was developed for detecting multiple types of polymorphisms in germplasm collections,such as single nucleotide polymorphism,small deletion and insertion etc.Rice is a very important food crop and a model plant for genome research also. There are complete genome sequence and a lot of other bioinformatics resources about it.So the markerassisted breeding is becoming more and more important in rice breeding. Some issues based on TILLING about identifying germplasm based on gene sequence,EMS mutagenesis breeding,developing functional marker in rice breeding in future were discussed.
