Cloning and Expression of Vibrio cholerae CTB Gene and the Recombinant CTB Protein Activation Assay
- VernacularTitle:霍乱弧菌CTB基因的克隆、表达及重组蛋白活性分析
- Author:
Guo-Guang ZHANG
;
Ya-Ming ZENG
;
Dong-Xiao LI
;
Hong-Xin ZHANG
;
Liang CHEN
;
- Publication Type:Journal Article
- Keywords:
Vibrio Cholerae CTB gene Expression of CTB gene GM1-ELISA
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
CTB protein possessed mucosal adjuvant immunoactivity. The CTB gene was amplified by PCR method from a strain V. cholerae. The nucleotide sequence of CTB gene was 375 bp and shared 96.0%~99.2% homology with other 6 CTB genes. The recombinant plasmid pTWIN1-CTB transformed E. coli strain BL21(DE3) expressed with 0.8 mmol/L IPTG. The molecular weight of expression products was identical with expectative weight by SDS-PAGE electrophoresis. The CTB fusion proteins mainly assembled inclusion bodies and the outputs of proteins were approximately 20% of the total bacterial proteins. The CTB proteins possessed mucosal immunoactivity by GM1-ELISA assay.
