Advances in Isobaric Tags for Relative and Absolute Quantitation Techniques Research
- VernacularTitle:同位素标记相对和绝对定量技术研究进展
- Author:
Zhi-Wen LUO
;
Liang ZHU
;
Wei-Fen XIE
;
- Publication Type:Journal Article
- Keywords:
Quantitative proteomics iTRAQ DIGE ICAT
- From:
China Biotechnology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Quantitative proteomics is a novel subject of proteomics research. There are several new techniques employed for the protein quantitative study. Isobaric tags for relative and absolute quantitation (iTRAQ) technology for protein quantitation using mass spectrometry is a recent powerful means of determining relative and absolute protein levels in up to four samples simultaneously. The iTRAQ reagent produced high quality, reproducible result in enriched complexes, organelles, and whole cell lysates. The status of the recent promising techniques and their possible future evolution were reviewed.
