Detection and Quantification of the Endophyte in Lolium perenne L.
- VernacularTitle:黑麦草内生真菌感染状况的检测及定量分析
- Author:
Dan SU
;
An-Zhi REN
;
Yu-Bao GAO
;
- Publication Type:Journal Article
- Keywords:
Lolium perenne L., Endophyte, Neotyphodium lolii, Real time PCR,
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A PCR assay used for detection and quantification of Neotyphodium lolii in tissues of the grass Lolium perenne L. was developed. Compared with the Microscopic analysis of stained tissues, this method was more accurate. For quantitative applications, real time PCR was used to quantify Neotyphodium lolii in Lolium perenne L.. The results showed that there were some variations between different individuals, but no variance existed between different tillers from the same plant. Thus it can be concluded that fungal content in the plant is not only associated with species/variety specific, but also with the genotype of the host plant as well.
