Selection of Cd~(2+) Binding-peptides and Their Affinities for Heavy Metal Ions
- VernacularTitle:Cd~(2+)结合肽的筛选及与重金属离子的亲和作用
- Author:
Jing-Shuang HUANG
;
Chun-Yan MA
;
Xin TONG
;
Zhuo-Lin YI
;
Liu XU
;
Can-Quan MAO
;
- Publication Type:Journal Article
- Keywords:
Metal binding-peptide,Phage random peptide library,Affinity chromatography,Selection,Cd~2+
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Selection and affinities of Cd binding peptides were assayed by phage random dodecapeptide library and affinity chromatography. Two Cd binding peptides were obtained, it was found that the affinities of Cu~ 2+ ,Co~ 2+ ,Zn~ 2+ ,Ni~ 2+ for Cd binding peptides were higher than that of Cd~ 2+ and Cr~ 2+ after detection of the amplified Cd binding peptides displayed phages to different heavy metal-charged resins; the detoxification of E.coli to Ni~ 2+ and Cd~ 2+ was enhanced when infected by Cd binding peptide displayed phages as compared with those of the control. The interaction of Cd binding peptide displayed phages with heavy metals charged resins was also observed under microscope. The work would be of great value and consequences for the study of interaction between heavy metals and proteins(peptides), as well as thedetoxification and bioremediation of heavy metals.
