Study on Purification and Identification of Streptavidin
- VernacularTitle:链霉亲和素纯化和鉴定方法的研究
- Author:
Fu-Ying LIU
;
Shu-Xia SONG
;
Long ZHENG
;
Huan-Ling ZHANG
;
Hong-Yu YOU
;
Jun-Xia WANG
;
- Publication Type:Journal Article
- Keywords:
Streptavidin, Purification and identification, Affinity chromatography
- From:
Microbiology
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this research is to refine the protocol of purification of SA and identify the character of SA. By utilizing the cold-denaturing method, most of other kinds of protein were screened out and SA was purified from the fermentation broth of L-183 by using the refined affinity chromatography method. The rate of recollection was checked to be 75%～85%. By identification, it is indicated that the molecular weight of self-made SA was 74.5kD, the biotin-combining number 3.2, the activity 11.2u/mg, the pI around 7.4. So, the essential characters of SA are same as described by documents.
