Isolation and purification of human coagulation factor Ⅶ from Cohn fraction Ⅲ paste
- VernacularTitle:从Cohn氏法组分Ⅲ沉淀纯化人凝血因子Ⅶ
- Author:
Shizhou XU
;
Qingrong ZHAO
;
Fangzhao LIN
;
Ling XIAO
;
Xiaopu XIAO
;
- Publication Type:Journal Article
- Keywords:
Cohn fraction Ⅲ precipitate;
Coagulation factorⅦ;
Isolation;
Purification
- From:
Chinese Journal of Blood Transfusion
2002;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and purify human coagulation factor Ⅶ from Cohn fraction Ⅲ precipitate.Methods The purification procedure of human factor Ⅶ from Cohn fraction Ⅲ precipitate involves dissolving fraction Ⅲ,absorbing factor Ⅶ onto barium citrate and eluting,ammonium sulfate fractionation and DEAE-Sepharose Fast Flow ion exchange chromatography,and Sephadex G-100 gel filtration chromatography.Results 10.1mg purified FⅦ was obtained from 400g Cohn fraction Ⅲ precipitate.The purified FⅦ has a specific clotting activity of 1775.8U/mg and the overall yield of FⅦ specific clotting activity is 17.6% of the starting material.The purity of FⅦ was judged by SDS-PAGE and there was only one protein band on the gel.Conclusion The procedure of purifying Ⅶ from Cohn fraction Ⅲprecipitate is established with satisfactory purity.
