Study of gene expression in osteoblasts induced shear stress
- VernacularTitle:剪切应力诱导成骨相关基因表达的研究
- Author:
Ding-Yu LI
;
Cheng-Kong CHENG
;
Zhengjian QIU
;
- Publication Type:Journal Article
- Keywords:
Osteoblasts;
Shear stress;
Mechanotransduction;
Bone formation-related gene
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the mechanotransduction pathways and gene expression in osteoblasts in response to fluid-induced shear stress in hope to be applied in tissue engineering.Methods Osteoblastic MG63 cells were exposed to shear stress of 12 dynes/cm2 for the designated times and their activations of MAPK(i.e., ERK,JNK,and P38)were examined by western blotting.The expressions of bone formation-related genes,in- cluding COX-2,Egr-1,c-fos,and OPN,were detected by RT-PCR.Results We found that shear stress induced phosphorylation of ERK,JNK,and P38 MAPK,and expressions of bone formation-related genes,including COX-2, Egr-1,c-los,and OPN.Conclusions Shear stress can turn on the signal transduetion and enhance bone forma- tion-related gene expression in osteoblasts.This phenomenon may imply that shear stress can be applied in tissue engineering.
