Osteogenic effects of induced autologous endothelial cells on bone marrow stromal cells of rabbits in co-culture condition
- VernacularTitle:联合培养时诱导的内皮细胞对自体骨髓基质干细胞成骨作用的影响
- Author:
Yuan SUN
;
Hong LIN
;
Zi-Zheng WU
;
Yu CHEN
;
Jian DONG
;
- Publication Type:Journal Article
- Keywords:
Tissue engineering;
Bone marrow stromal cells (BMSCs);
Osteoblasts;
Endothelial cell(EC);
Co-culture
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the osteogenic effects of induced endothelial cell(EC)on bone marrow stromal cells(BMSCs)of rabbits in co-culture condition.Methods BMSCs were obtained from rabbits by density gradient centrifugation.The adhesive ceils were preserved to passage in culture.The cultured cells were divided into four groups:group A(BMSCs),group B(BMSCs osteogenic induction),group C(EC induction)and group D (co-culture of induced BMSCs and EC).The cell morphology,immunofluorescence,cell proliferation,alkaline pbosphatase(ALP)activity,osteocalcin synthesis were observed to determine the effects of induced autologous EC on the osteogenic potential and cellular compatibility of BMSCs.Results The immunochemical staining showed that the BMSCs were induced into EC in group C.The cellular compatibility of BMSCs and EC was good.The ALP activity and osteocalcin content were obviously higher in group D than in any other groups(P＜0.05).The cell proliferation difference was not obvious between groups(P＞0.05).Conclusions The cellular compatibility of induced osteoblasts and induced EC is perfect.The ECs can significantly increase the viability and ALP activity of induced osteoblasts.
