The effect of long-term culture in vitro on biological characteristics of human adipose tissue derived stem cells
- VernacularTitle:长期体外培养对人脂肪源性间充质干细胞生物学特性的影响
- Author:
Hui-Wu LI
;
Ke-Rong DAI
;
Ting-Ting TANG
;
Shengli XIA
;
Yaokai GAN
;
Yuanqing MAO
;
- Publication Type:Journal Article
- Keywords:
Adipose-derived stem cells (ADSCs);
Biology;
Culture in vitro;
Human
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of lnng-term in vitro culture on the biological properties of adipose-derived stem cells(ADSCs)as seeding cells of tissue engineering.Methods The surface makers and apoptosis of primary and passaged human ADSCs were identified by flow cytometric analysis.Osteogenic differentiation of ADSCs at different passages were identified by alkaline phosphatase(ALP),Von Kossa staining and RT-PCR respectively.Results The surface marker expression of mesenchymal stem cells on ADSCs was high and did not change with passages of the cells.The early apoptosis rate of the cells was 1% to 2%,and increased insignificantly from passage one to passage nine.The osteogenic potential of ADSCs confirmed by ALP,Von Kossa staining and RT-PCR was maintained to as late as passage eight.Conclusion Since the biological properties of ADSCs are stable,they can be served as optimal seeding cells for tissue engineering and regenerative research.
