Effects of type I collagen on adhesion,proliferation and osteogenic differentiation of rabbit bone marrow stromai cells on PLGA-[ASP-PEG]scaffolds
- VernacularTitle:Ⅰ型胶原在PLGA-[ASP-PEG]表面修饰对兔骨髓基质干细胞生物力学的影响
- Author:
Hai-Tao PAN
;
Qi-Xin ZHENG
;
Xiao-Dong GUO
;
Yong LIU
;
Yulin SONG
;
- Publication Type:Journal Article
- Keywords:
Polylactic glycolic acid/aspartic acid-polyethylene glycol(PLGA-[ASP-PEG]);
Type I collagen;
Osteogenic differentiation;
Bone marrow stromal cells(BMSCs);
Biomechanics
- From:
Chinese Journal of Orthopaedic Trauma
2004;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of surface modification of PLGA-[ASP-PEG] scaffold with typeⅠcollagen on the adhesion,proliferation and osteogenic differentiation of rabbit bone marrow stromal cells (BMSCs).Methods After PLGA-[ASP-PEG] materials were modified with typeⅠcollagen chemically,the collagen was coated onto the materials physically.The BMSCs obtained from rabbits were cultured on the modified PLGA-[ ASP-PEG] and on the unmodified PLGA-[ ASP-PEG] as control.The adhesion and proliferation behavior of the cells was analyzed and the expressions of osteogenie marker alkaline phosphatase,osteocalcin,osteopontin,typeⅠcollagen and core binding factor al were also detected.Results X-ray photoelectron spectrometry(XPS) confirmed that TypeⅠcollagen was grafted onto the surface of PLGA-[ASP-PEG] successfully and the collagen content on the materials modified chemically and physically was significantly increased.The abilities of adhesion and proliferation and the expressions of osteogenie makers of the BMSCs were significantly greater than those in the control group(P＜0.05).Conclusion Since Type collagen I can improve the biocompatibility of PLGA- [ASP-PEG] scaffold materials,it can be used as a new way to optimize scaffolds in tissue engineering.
