The Research on Expression and Purification of a Chimeric Anti-p185 Antibody
- VernacularTitle:抗P185工程抗体表达与纯化研究
- Author:
Jing-Juan QIAO
;
Hai-Bo LIU
;
Juan-Juan ZHU
;
Lian-Sheng CHENG
;
Jing LIU
;
- Publication Type:Journal Article
- Keywords:
Chimeric antibody Affinity purification Cation exchange purification Stability
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The Her-2 proto-oncogene encodes a 185kDa transmenbrane glycoprotein p185 which has intrinsic tyrosine kinase activity. It is overexpressed in several malignant human tumors like breast cancer. A chimeric antibody by assembling a single-chain Fv antibody and a human IgG1 Fc fragment was constructed. This chimeric antibody reacts with tumor surface antigen p185c-erbB-2 specifically. In order to put the antibody into clinical application, two steps purification method was used to attain the antibody’s purity more than 95%. Both the lyophilized pharmaceutical formulations of the antibody were found. The formulations can keep the stability and activity of the antibody for at least one year. These results were the foundation of the chimeric antibody for cancer therapy.
