Plasmid-mediated carbapenemase KPC-2 in a strain of Klebsieila pneumoniae
- VernacularTitle:发现一株产KPC-2型碳青霉烯酶肺炎克雷伯菌
- Author:
Xing-Guo ZHANG
;
Xiao-Xing DU
;
Rong ZHANG
;
Ze-Qing WEI
;
Yun-Song YU
;
Ya-Gang CHEN
;
Lan-Juan LI
;
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
Plasmids;
Enzymes
- From:
Chinese Journal of Laboratory Medicine
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistant mechanism of imipenem-resistant K. pneumoniae.Methods The minimal inhibitive concentrations (MICs) of the antimicrobial agents were determined by Etest.Isoelectric focusing electrophoresis (IEF),plasmid extraction,conjugation, transformation,PCR amplification,cloning and sequencing were carried out for analyzing the encoding gene of ?-1actamases.Results Three kinds of ?-1actamases were detected with pIs of 7.2,6.7,and 5.4.in a clinical strain of K.pneumoniae.These ?-1actamases were TEM-I (pI,5.4),SHV-12 (pI,8.2) and KPC-2 ( pI,6.7 ) confirmed by sequencing of the PCR products.Only one band of ?-1actamase with pI 6.7 was displayed in the transformant.A 1500 bp segment,which contained the KPC-2 gene confirmed by nucleotide sequence analysis,was cloned from a 60 000 bp plasmid of the transformant.Conclusion The strain of K.pneumoniae resistant to imipenem produces a plasmid-mediated carbapenemase KPC-2 which belongs to Bush group 2f,class A ?-1actamase.
