Cell Cylce Regulatory Effects of Cyclic AMP in Cancer Cells Which Lack Wild-type p53.
- Author:
Joung Soon JANG
1
;
Joung Hun KANG
;
Byung Kiu PARK
;
Sang Gu HWANG
Author Information
1. Department of Internal Medicine, Gyeongsang National University College of Medicine and Gyeongsang Institute of Cancer Research, Chinju, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
Dibutyryl-cAMP;
Cell cycle regulation
- MeSH:
Antigen-Antibody Complex;
Blotting, Northern;
Blotting, Western;
Breast Neoplasms;
Cell Cycle Proteins;
Cyclic AMP*;
Cyclic AMP-Dependent Protein Kinases;
Cyclin D3;
Down-Regulation;
Immunoprecipitation;
Phosphotransferases;
Prostate;
Retinoblastoma Protein;
RNA, Messenger;
S Phase
- From:Journal of the Korean Cancer Association
2000;32(4):750-756
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The activator of protein kinase A, cyclic AMP, has been a recognized growth inhibitor of certain cell types. The present study aimed to investigate the effects of dibutyryl cAMP on the growth of cancer cells which lack wild-type p53 and to determine the mechanism of growth inhibition. MATERIALS AND METHODS: Prostate and breast cancer cells were treated with dibutyryl cAMP and compared with untreated cells. Growth patterns of cells were assessed by trypan blue-excluding method and western blot was done to determine protein levels of cell cycle regulatory proteins which govern G1 and G1/S phase. Northern blot and immunoprecipitation were done to determine the level of mRNA of p21 and the association between cell cycle regulatory proteins. In vitro immune complex kinase assay was done to assess the activity of cdk2. RESULTS: cAMP reduced cell growth by 48 h. Cyclin D3 level was downregulated and RB protein level was decreased and mostly unphosphorylated forms remained. The association of RB with E2F1 was increased. While cdk2 levels remained constant throughout cAMP treatment, the activity of cdk2/cyclin E complex, which is responsible for entry into S phase, was downregulated. Cdk inhibitors, p27 and p21 were induced with cAMP treatment. CONCLUSION: These observation suggest that the growth inhibitory effects of dibutyryl cAMP on prostate and breast cancer cells were mediated by induction of cdk inhibitors such as p21 and p27 and RB activation in accordance with downregulation of cdk2.
