Study on the Bombardment Factors for Kentucky Bluegrasss (Poa pratensis L.)
- VernacularTitle:影响草地早熟禾(Poa pratensis L.)基因枪转化的关键因素研究
- Author:
Lie-Bao HAN
;
Jin-Na XIN
;
Jun LIU
;
Hui-Ming ZENG
;
- Publication Type:Journal Article
- Keywords:
Bluegrass DREB1A Biolistic bombardment
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Embryonic callus of Kentucky bluegrass were used as material for genetic transformation with the DREB1A gene by particle bombardment. Parameters of biolistic bombardment were studied. The optimal methods showed as follows: plasmid DNA are coated by Ca(NO_3)_2 and PEG4000, 1?m golds are the vectors of plasmid DNA, 6 cm bombardment height distance, 1 time and without osmotic treatment are favorable to transgenic efficiency. The concentration hygromycin (Hy) , which is the selection mark for cultivar‘Baron’ is 100mg/L.
