Transformation of Kentucky Bluegrasss (Poa pratensis L.) by Particle Bombardment
- VernacularTitle:基因枪转化法获得草地早熟禾(Poa pratensis L.)转基因植株
- Author:
Jin-Na XIN
;
Lie-Bao HAN
;
Jun LIU
;
Xiu-Bin HAN
;
- Publication Type:Journal Article
- Keywords:
Bluegrass BADH-CMO double-gene DREB1A gene CMO gene
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
BADH-CMO double gene,CMO gene and DREB1A gene were transformed respectively to embryonic callus of Kentucky bluegrass by particle bombardment. Then the embryonic callas of kentucky bluegrass were put in meclium for subculture which is mixed with 100mg/L hygromycin and the meclium for plantlet regeneration which mixell with 50mg/L hy gromycin about one month. Thus,the hygromycin-selectecl plants were obtained and were transplantecl into tlowerpots. The results of the PCR and Southern blot analysis indicated that the DREB1A gene,CMO gene and BADH-CMO double-gene were integrated into the genomic DNA of Kentucky bluegrass.
