Heterologous Expression of Zygosacharomyces rouxii ZrGpd 1 in Pichia farinosa
- VernacularTitle:鲁氏酵母3-磷酸甘油脱氢酶基因(ZrGPD1)在粉状毕赤酵母中的表达
- Author:
Jie ZHANG
;
Xian-Zhong CHEN
;
Wei SHENG
;
Xue-Ming TANG
;
Ge-Jian ZHU
;
- Publication Type:Journal Article
- Keywords:
Glycerol Glycerol 3-phosphate dehydrogenase Osmotolerant yeast Zygosaccharomyces rouxii Integrative expression
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To examine the effects of heterologous expression of ZrGPD1 (encoding glycerol 3-phosphate dehydrogenase ) cloned from osmotolerant yeast Zygosacharomyces rouxii on glycerol production in wild Pichia farinosa,the URA3 gene was amplified from P. farinosa as the homology integrative region. A recombinant plasmid (pUR-ZG) was constructed then transformed into P. farinosa by electroporation. The transformant pfa-gu was obtained by the selectable marker Zeocin TM . Primary results showed that the biomass of pfa-gu was higher than the wild type in the flask and after 72h fermentation the concentration of glycerol of pfa-gu was 37g/L enhanced 30% in comparison with the wild type. It is concluded that heterologous expression of ZrGPD1 is useful for increasing glycerol production and the ability of osmoregulation in P. farinosa.
