Analyzing Role of △~5 Desaturase in Biosynthesis of Arachidonic Acid Using Real Time PCR
- VernacularTitle:实时PCR分析△~5脱饱和酶在花生四烯酸合成中的作用
- Author:
Jing XIAO
;
Zhi LIU
;
Min ZHU
;
Long-Jiang YU
;
Lu ZHU
;
Peng-Peng ZHOU
;
- Publication Type:Journal Article
- Keywords:
?~5 desaturase Arachidonic Acid Real time PCR Mortierella alpina
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Arachidonic acid is an essential fatty acid in human nutrition and a biogenetic precursor of the biologically active prostaglandins and leukotrienes. ?~5 desaturase catalyzes the ?~5 dehydrogenation of di-homo-?-linolenic acid to form arachidonic acid in biosynthetic pathway of arachidonic acid. Using real time PCR technology,the transcriptional expression levels of gene encoding ?5 desaturase in three Mortierella alpina strains M10,M6 and M23,and in different growth phase of high arachidonic acid yielding strain M6,were determined. Results showed that there was a distinct corelationship between mRNA transcript level of ?~5 desaturase gene and biosynthesis of arachidonic acid. Results indicated that ?~5 desaturase plays an important role in arachidonic acid biosynthesis.
