Research of Desulfhydrase Involved in L-cysteine Biosynthetic Pathway in Pseudomonas sp.
- VernacularTitle:假单胞菌生物合成L-半胱氨酸途径中脱巯基酶的研究
- Author:
Yang-Sheng YU
;
Yang LI
;
Yong-Jie JIN
;
Gang BAI
;
Wen-Bo YANG
;
- Publication Type:Journal Article
- Keywords:
L-cysteine desulfhydrase, Pseudomonas sp., Gene cloning, Gene expression, Enzymology characterization
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The L-cysteine desulfhydrase gene (cd) of Pseudomonas sp.TS1138 was amplified by PCR,and the amplified gene was recombined in the cloning vector pBluescript SKII.The 1.2kb DNA fragment containing cd was sequenced,and its homology with other desulfhydrases was blast; then the cd was cloned into the expression vector pET-21a(+), and afterward expressed by IPTG inducement.The expression protein was purified by Ni-NTA His-Bind Resin.Then the expression protein was identified by the method of activity staining of desulfhydrase, and the characterization of L-cysteine desulfhydrase and the critical role it played in the L-cysteine biosynthetic pathway were discussed.
