Screening Citrinin Mutants from the Transformants Library of Monascus ruber M-7 by Agrobacterium-mediated DNA transfer
- VernacularTitle:红曲霉T-DNA插入转化库中桔霉素突变子的筛选
- Author:
Yue-Di DING
;
Yan-Chun SHAO
;
Yi-Ping XU
;
Fu-Sheng CHEN
;
- Publication Type:Journal Article
- Keywords:
Monascus ruber,Citrinin,HPLC,Agrobacterium-mediated DNA transfer
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
200 citrinin mutants were screened with the inhibition zone method from the transformants library of Monascus ruber M-7 by Agrobacterium-mediate DNA transfer, which contains more than 5,000 transformants.Then 53 mutants, whose citrinin contents ranged from 0.04?g/g to 154.57?g/g in the red fermented rice (RFR), were achieved by high performance liquid chromatography (HPLC).Color values of RFR prepared by these mutants were also detected.The results showed that there was a positive correlation between the citrinin content and the color value among the mutants.These results provide materials and research bases for ferrther studying the relationship between the production of citrinin and pigment of Monascus ruber at molecular level.
