Effect of Fusion Protein Cleavage Site Mutations of NDV ZJ1 Strain on Its Fusion Activity
- VernacularTitle:新城疫病毒ZJ1株F蛋白裂解位点突变对其融合活性的影响
- Author:
Yan-Mei ZHANG
;
Shun-Lin HU
;
Qing SUN
;
Yan-Tao WU
;
Xiu-Fan LIU
;
- Publication Type:Journal Article
- Keywords:
Newcastle disease virus, F protein,Mutation,Fusion activity,Cleavage activity
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
NDV strain ZJ1 strain , a highly virulent NDV strain, has been prevalent among the waterfowls in China mainland in the past years. Multi-basic amino acid sequence distribute in the protease cleavage site of F protein of this strain. Recombinant expressing plasmid pCI-FT, was generated by converting multi-basic amino acid sequence of 112, 115, 117 of the protease cleavage site of F_ 0 protein, to the non-basic amino acid sequence characteristic of avirulent NDV strain. The result from co-expression of mutant or parental F protein with homologous HN protein in COS-1 cells revealed that both mutant and parental F protein had fusion activity. The result from co-expression of mutant or parental F protein with homologous HN protein in CEF cells showed that the cleavage activity of mutant F protein was significantly reduced. The study built a foundation for mutagenesis of amino acid sequence of the protease cleavage site of F_ 0 protein at the full-length cDNA clone level, study on factors contributing to virulence and construction of candidate vaccine strain, and so on.
