Cloning and Analysis of Genes Correlated to Trehalose Biosynthesis from Micrococcus luteus
- VernacularTitle:藤黄微球菌海藻糖生物合成基因的克隆与鉴定
- Author:
Xue-Feng HUANG
;
Li-Ming OUYANG
;
Hai-Zheng WU
;
Jiang YE
;
Hui-Zhan ZHANG
;
Qin-Sheng YUAN
;
- Publication Type:Journal Article
- Keywords:
Micrococcus luteus, Trehalose, Maltooligosyl trehalose synthase (MTSase), Maltooligosyl trehalose trehalohydrolase (MTHase), Homologous analysis
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Genes related to trehalose biosynthesis from a bacterial strain Micrococcus luteus which can convert partially hydrolyzed starch into trehalose were cloned.Full sequence of gene (MtreY) encoding trehalose maltooligosyl trehalose synthase (MTSase) and partial sequence of gene (MtreZ) encoding maltooligosyl trehalose trehalohydrolase (MTHase) were got using PCR combined non-random shotgun method.Sequence analysis of MtreY predicts a 2370bp open reading frame encoding a protein of 790 amino acids with a predicted molecular weight of 86734 Da.Homologous analysis shows that this new gene has the same conservative motifs with ?-amylase family enzymes.The MtreY gene was expressed in E.coli, and the expression product has the anticipative enzyme activity.