Study on Rapid Methods for Quantitative Analysis Rhamnolipid and Its Influence Factors
- VernacularTitle:鼠李糖脂快速定量分析方法及其影响因素研究
- Author:
Guo-Man LU
;
Hong-Yu LIU
;
Guang-Ming ZENG
;
Guo-He HUANG
;
Hui ZHANG
;
- Publication Type:Journal Article
- Keywords:
Rhamnolipid,Pseudomonas aeruginosa,H_ 2 SO_ 4 -anthrone analysis method,Quantitative analysis
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to find an easy and rapid quantitative analytical method to detect rhamnolipid produced by Pseudomonas aeruginosa, three methods, H_ 2 SO_ 4 -anthrone analysis method, L-cysteine-H_ 2 SO_ 4 method and phenol-H_ 2 SO_ 4 method, were compared in the present paper, and the influence factors were also considered.The results showed that H_ 2 SO_ 4 -Anthrone analysis method was better than the others and its optimal reaction condition was obtained.The influence to the quantitative analysis of rhamnolipid from the residual glucose and the top clean liquid layer in the ferment solution could be ignored.But the influence from the bacterial body and the middle layer of the ferment solution reached a certain degree.Thus, the bacterial body should be removed before measuring.However, the influence from the middle layer of the ferment solution could be avoided by making a standard curve which was made by using a rhamnose mixed with the middle layer ferment solution.
