PCR Detection of N.coenophialum and N.lolii
- VernacularTitle:Neotyphodium coenophialum和N.lolii的PCR检测
- Author:
Yue-Ting LIU
;
Fang LIAO
;
Tie-Jun CUI
;
Guo-Ming HUANG
;
Jia-Feng LUO
;
- Publication Type:Journal Article
- Keywords:
N.coenophialum, N.lolii, PCR, Detection, Single seed
- From:
Microbiology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
18 fungal strains including N.coenophialum,N.lolii, N.huerfanum、N.chisosum、N.aotearoae、N.sp.and 8 varieties of grass seeds belonging to Festuca arundinacea and Lolium perenne have been studied.With amplification of IS1～IS3 and F1～R1 of genomic DNA, the primers Tub-2-F～Tub-2-R from Tubulin-2 gene and F3～R3 from NC25 gene have been designed.A PCR method to detect N.coenophialum and N.lolii was established, and also a nested-PCR method to detect N.coenophialum and N.lolii in single seed was established.These PCR detection methods are strongly special and much credible and rapid-speeded.
