Repetitive element-based polymerase chain reaction to track the nosocomial infec- tions caused by Staphylococcus aureus
- VernacularTitle:重复序列引物聚合酶链反应追踪金黄色葡萄球菌所致医院感染
- Author:
Ge HUANG
;
Ting DONG
;
Tieying HOU
;
Li-Yan ZHANG
;
Mei WANG
;
Ka-Bin RONG
;
- Publication Type:Journal Article
- Keywords:
Repetitive element-based polymerase chain reaction;
Staphylococcus aureus;
Nosocomial infection
- From:
Chinese Journal of Infection and Chemotherapy
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The technique of repetitive element-based polymerase chain reaction(rep-PCR)was used to track an epi- demic of nosocomial infection caused by Staphylococcus aureus in our hospital.Methods The 50 S.aureus isolates were identi fled by PHOENIX-100 automatic Microbiological Identification System.Oxacillin-salt-supplemented agar was used to screen methicillin-resistant S.aureus(MRSA)phenotype.The resistant gene mecA was tested by PCR.The technique of rep-PCR was applied to type S.aureus isolates.Results The mecA gene was identified in 22 of the 50 S.aureus isolates.Nineteen of the 22 strains were isolated from patients.Nine to eleven bands were observed in electrophoretic pattern of all the 50 S.aureus iso- lates by rep-PCR under the conditions of this study.These strains were accordingly classified into 11 different genotypes.Con- clusions The rep-PCR technique is a rapid,simple and reliable genotyping method.It is an ideal tool to track the source of noso- comial infections at molecular level.
