Correlation between Genetic Polymorphism of CYP2D6 and CYP1A1 and Susceptibility of Renal Cell Carcinoma in Korean.
- Author:
Kyu Wook PARK
1
;
Se Il JUNG
;
Gyung Woo JUNG
;
Heon Young KWON
;
Jin Sook JEONG
;
Jin Ho CHUN
;
Jin Han YOON
Author Information
1. Departments of Urology, Dong-A University College of Medicine .
- Publication Type:Original Article
- Keywords:
Renal cell carcinoma;
CYP2D6;
CYP1A1;
Genetic polymorphism
- MeSH:
Alleles;
Carcinoma, Renal Cell*;
Cytochrome P-450 CYP1A1*;
Cytochrome P-450 CYP2D6*;
DNA;
Genotype;
Odds Ratio;
Polymerase Chain Reaction;
Polymorphism, Genetic*;
Polymorphism, Restriction Fragment Length
- From:Journal of the Korean Cancer Association
2000;32(4):801-809
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Many of the enzymes handling environmental factors are polymorphic and may confer variable susceptibility to renal cell carcinoma (RCC). Among those, the author studied genetic polymorphisms of CYP2D6 (B & T) and CYP1A1 in RCCs and controls in Korean. MATERIALS AND METHODS: Using 132 RCCs and 94 controls, first PCR products were obtained in 104 RCCs and 94 controls with CYP2D6, and 74 RCCs and 56 controls with CYP1A1. Res triction enzyme - BstN I/EcoN I for CYP2D6 (B & T), and NCo I for CYP1A1-digestion was followed to analyze constitutive DNA. RESULTS: In both RCCs and controls, no mutant allele of CYP2D6 (B & T) was detected and the susceptibility for occurrence of RCC was unable to evaluate. With CYP1A1 RFLP, homozy gous wild type (WW) was seen in 68 (52.3%; 37 RCCs, 31 controls), heterozygous mutant type (WM) in 54 (41.5%; 32 RCCs, 22 controls) and homozygous mutant type (MM) in 8 (6.2%; 5 RCCs, 3 controls). The odds ratios (95% CI) of RCC susceptibility for CYP1A1 genotype were 1.15 for WM and 1.36 for MM. Even though not significant statistically, higher tendency in MM presented. CONCLUSION: There is no association between susceptibility for the occurrence of RCC and genetic polymorphism of CYP2D6 (B & T) and CYP1A1.
