Fetal Membrane Derived Adherent Cells: a Novel Source for Mesenchymal Stem Cells
- VernacularTitle:胎膜组织来源间质干细胞的研究
- Author:
Zhi-Cheng MA
;
Zhi-Gao LONG
;
Ling-Qian WU
;
Qian PAN
;
De-Sheng LIANG
;
He-Ping DAI
;
Kun XIA
;
Jia-Hui XIA
;
- Publication Type:Journal Article
- Keywords:
Fetal membrane derived adherent cells Mesenchymal stem cells Fetal membrane Induced differentiation
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for isolation and purification of fetal membrane derived adherent cells (FMDACs) , and investigate their biological characteristics. Method: FMDACs were isolated with trypsin inducing and cultured in vitro. FMDACs were induced to differentiate into osteoblasts and adipocytes. FACS and immunocytochemistry technique were used to examine the cell surface antigen. The genetic stability was verified by karyotype analysis. Results: FMDACs were successfully isolated and expanded in vitro. They had strong proliferative ability. FMDACs were positive for CD44 and CD29, but negative for CD34, CD14 and CD45. FMDACs were differentiated into osteoblasts and adipocytes after inducement. The karyotype was stable in the sixth-passaged FMDACs and the tumorigenicity was not found. Conclusion; FMDACs have the possibility of multipotent stem cells, which have strong capacities of self-renewal and multidirectional differentiation. The genetic background of FMDACs is stable. FMDACs may be used as a kind of novel seed cells for tissue engineering.
