DNA Shuffling of Arabidopsis thalianna K+ Uptake Transporter Gene
- VernacularTitle:拟南芥K~+转运蛋白AtKup1基因的DNA改组
- Author:
Zhao-Kui GUO
;
Qian YANG
;
Quan-Hong YAO
;
Xiu-Qing WAN
;
Pei-Qiang YAN
;
- Publication Type:Journal Article
- Keywords:
AtKup1 gene DNA shuffling trk1△trk2△Yeast mutant
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
The DNA fragment sized 2 139bp, the same Sequence with AtKup1 gene from Arabidopsis thalianna was used as the templates for DNA family shuffling. The shuffeld AtKup1 gene library was expressed in the mutant of 5. cerevisae in which potassium transporter gene TRK1 and TRK2 were knocked out by homologous recombination. Then the screening was carried out in the low potassium media containing 5. 0 mmol/L KC1 and no histidine in it. it was found that both of diverse and wild AtKup1 gene can rescues the trk1△trk2△yeast mutant strain in low [ K + ] medium. The growth of 2 clones yeast containing diverse AtKup1 were beter than that of AtKup1 wild gene transformant. The sequencig results of the shuffeld AtKup1 showed that there were 2 nucleotide changed, which resulted in 2 amino acid variations in it compared with the original AtKup1. The potassium uptaking capacity of shuffled AtKup1 gene increased significantly when it was transformed into tobacco.
