Secreted Expression of M annanase Gene in Pichia pastoris and Anylysis of Enzymic Properties
- VernacularTitle:甘露聚糖酶基因在毕赤酵母中的表达及酶学性质研究
- Author:
Yu QIAO
;
Xiao-Bing CHEN
;
Hong-Biao DING
;
Ming YUE
;
- Publication Type:Journal Article
- Keywords:
Mannanase Pichia pastoris Expression
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
A PCR method was used to amplify the sequence encoding the mature peptide of?-mannanase of Bacillus subtilis. The gene was inserted into the Pichia pastoris vector pPIC9K, downstream of?-factor signal peptide sequence. The resultant recombinant plasmid pPIC9K-MAN was lineared by BglII digestion and introduced into the host Pichia pastoris GS115 by PEG method. After screen, the recombinant P. pastoris strain MAN22 was obtained and fermented in large scale 5L fermenter. The recombinant mannanase activity could reach to 1102IU/ml. The properties of the recombinant mannanase were characterized.
