Cloning and Characterization of Swamp Buffalo SRY Gene
- VernacularTitle:水牛SRY基因的克隆及序列分析
- Author:
Qiang FU
;
Ming ZHANG
;
Wen-Song QIN
;
Hai-Ying ZHENG
;
Ke-Huan LU
;
- Publication Type:Journal Article
- Keywords:
Buffalo SRY Southern blot
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
The SRY gene from buffalo (Bubalus bubalis) genome was amplified by the polymerase chain reaction ( PCR) with primers based on the sequence of Hostein SRY gene. The amplified fragment was 2005 bp include 5UTR ( 1 - 504bp) and 3'UTR(1196 - 2005bp). And the amplified fragment was cloned and sequenced. Sequence analysis showed that the coding region of SRY gene (505 - 1195bp) from buffalo was highly homologous with those of other bovine counterpart genes (96% homology) , especially in the HMG box region (99%homology). It was found that there were only signal on male buffalo genome on Southern blot,which indicate SRY gene are highly conservative on evolves.
