Cloning, Sequencing of Suaeda heteroptera kitag CMO cDNA and Construction of its Recombinant Plant Expression Vector
- VernacularTitle:翅碱蓬CMO cDNA基因克隆、测序及重组植物表达载体的构建
- Author:
Chong-Bin ZHONG
;
Chang-Jiang LIU
;
Teng FEI
;
Xiao-Dong YUAN
;
Li-Hui SUN
;
- Publication Type:Journal Article
- Keywords:
Choline monooxygenase pMD18-T-simple vector pBI121 plant expression vector E. coli JM109
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Total RNA was extracted from leaf of Suaeda hetroptera kitag, then the CMO ( choline monooxygenase) cDNA was amplified using the reverse transcriptase polymerase chain reaction ( RT-PCR) method and cloned into pMD-T-simple vector. The positive clones from the Blue/White Screen were sequenced. After confirming its validity, the CMO gene fragment was cloned into pBI121 vector. Double enzyme restriction and PCR analysis indicated that the pBI121/CMO recombinant plasmid was successfully constructed.
