Development and Application of RNAi Interference Library in Functional Genomics
- VernacularTitle:RNA干扰文库在功能基因组学研究中的发展及应用
- Author:
Ying ZHANG
;
Yao-Wu YANG
;
Jian-Wei WANG
;
Jian-Guo QU
;
Tao HONG
;
- Publication Type:Journal Article
- Keywords:
RNAi library Functional genomics High-throughput screening
- From:
China Biotechnology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
RNA interference ( RNAi) is a process in which double-stranded RNA ( dsRNA) induces specific postranscriptional silencing of homologous transcripts. Systematic silencing of genes on a genome-wide scale using RNAi library targeting many thousands of genes provides a powerful research tool in functional genomics. RNAi libraries, which may be derived from plasmids cloning, virus packaging, PCR amplification, chemically synthesis or enzymatic digestion, have been successfully used to identify gene function, dissect signaling pathway and discover drug targets, etc. Promising progresses have been made in this field. The development, application and problems of RNAi library methodology and future prospects were reviewed.
