A High-throughput and Quantitative Assay Based on Fluorescence Intensity for Detection of Apoptosis
- VernacularTitle:一种基于荧光强度分析的高通量定量检测细胞凋亡方法
- Author:
Ling-Ling YE
;
Hong LIU
;
Xing-Mao LIU
;
Shi-Chong LI
;
Ben-Chuan WU
;
Qi-Wei WANG
;
Zhao-Lie CHEN
;
- Publication Type:Journal Article
- Keywords:
Fluoresence intensity High throughput Quantitative assay Apoptosis
- From:
China Biotechnology
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the different permeability of DNA-intercalant dyes YO-PRO-1(YP) and propidium iodide (PI) to the membrane of viable, apoptotic and necrotic cells, cell samples were stained with 4?mol/L YP and 4?g/ml PI for 10 min, and the fluorescence intensity of both YP and PI were measured by fluorometer at Ex/Em wavelength of 485/538nm and 530/590nm, respectively. The correlation between YP fluorescence intensity and the apoptotic cell number was confirmed by fluorescence microscope and linear regression(r=0.999,P
