Use of 16S-rRNA Hybridization Probes to Detect the Cellulolytic Bacteria in Rumen
- VernacularTitle:利用定量杂交法检测绵羊瘤胃纤维细菌的研究
- Author:
Hai-Rong WANG
;
Xian-Zhi HOU
;
Ai-Wu GAO
;
Li-Hua LV
;
Yuan GUO
;
- Publication Type:Journal Article
- Keywords:
16S rRNA, Oligonucleotide probe, Quantified hybridization, Ruminal bacteria
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the general bacterial probe and specific cellulolytic bacterial probes were used to quantify the bacteria in rumen. The total RNA were extracted and then hybridized with general bacterial probe after a dilution of concentration. The result showed that there was a high correlation between the hybridization signal and the dilution of total bacterial RNA. Based on the result above, the quantities of three cellulolytic bacteria in rumen sample were detected. The comparative RNA percentage of three cellulolytic bacteria to total bacterial RNA were similar to the previous reports. It can be concluded that the quantification of bacteria in rumen could be conducted by this approach, and which could be used in future research.
