Rapid Characterization of Recombination Clone by PCR Screening of Individual Bacterial Colonies
- VernacularTitle:单菌落PCR法直接快速鉴定重组克隆
- Author:
Shu-Xia CHEN
;
Xiao-Wu WANG
;
Yu-Lin FANG
;
- Publication Type:Journal Article
- Keywords:
Individual bacterial colonies PCR, GFP, LTB-ST, Recombination clone, Screening
- From:
Microbiology
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The recombination clones contained CFP, LTB-ST foreign gene were screening by PCR using individual bacterial colonies as template, the aimed band was amplified from positive clones, the result was as well as plasmid PCR. The selecting of agrobacterium transformed with recombination plasmid could also use this method of PCR screening of individual bacterial colonies. The result of individual bacterial colonies PCR was as well as that of PCR using bacterial solution as template. It showed that the method individual bacterial colonies PCR was an efficient, easy one that characterized recombination clones.
